Role of the cAMP-PKA-CREB-BDNF pathway in abnormal behaviours of serotonin transporter knockout mice.

Serotonin transporter gene-linked polymorphic region polymorphisms are associated with anxiety, neuroticism, affective disorders and vulnerability to stressful life events; however, the relevant physiological mechanisms are not well understood. Serotonin transporter knockout mice have been widely used as a model of allelic variation of serotonin transporter function in humans; herein, wild-type mice and heterozygous and homozygous knockout mice models were established to explore the behavioural changes related to different genotypes and the possible physiological mechanisms. Behavioural changes were assessed using behavioural tests, namely, elevated plus maze, open field, Morris water maze and rotarod tests. Serum indicators were detected using the enzyme-linked immunosorbent assay. Compared with wild-type mice, homozygous mice showed significant anxiety-like behaviours in the plus maze and open field tests; conversely, anxiety-like behaviours in heterozygous mice were less pronounced. Homozygous mice also showed cognitive impairment and motor inhibition in the Morris water maze and rotarod tests. Serotonin levels decreased in both heterozygous and homozygous mice, and 5-hydroxytryptophan, protein kinase A, adenylyl cyclase, cyclic adenosine monophosphate response element-binding protein and brain-derived neurotrophic factor levels were lower in homozygous mice than in wild-type and heterozygous mice, whereas no statistical differences were found between wild-type and heterozygous mice. Additionally, there was a correlation between serological and behavioural indicators. This study provided experimental evidence that the cyclic adenosine monophosphate-protein kinase A-cyclic adenosine monophosphate response element-binding protein-brain-derived neurotrophic factor pathway may be involved in the regulation of polymorphism to stress and enriched the behavioural and physiological characteristics of serotonin transporter knockout mice.

The association between demographic characteristics, personality, and mental health of bus drivers in China: A structural equation model.

BACKGROUND: The psychological status of drivers is related to adverse driving behaviors. Although there are several studies on the mental health and traffic accidents among bus drivers, related studies on the association between mental health and individual characteristics are scarce. METHODS: Our report is on a cross-sectional study of bus drivers that took place between October 2014 and May 2016. A total of 596 bus drivers who worked for transportation companies and underwent occupational physical examinations at The Guangxi Zhuang Autonomous Region workers' Hospital during the study period were included. Demographic characteristics were collected by a self-designed demographic questionnaire. The Eysenck Personality Questionnaire (EPQ) and the Symptom Checklist 90-Revised (SCL-90-R) were used to evaluate the personality and mental health of bus drivers, and the results of the scales were compared with Chinese norms. The structural equation model was used to analyze the correlation among demographic characteristics, personality characteristics, and mental health. RESULTS: Except for the lie dimension, the average scores of the driver's personality dimension were higher than the Chinese norm, and the means of somatization and phobia scores of bus drivers were also higher; obsessions-compulsions, interpersonal sensitivity, and depression scores were lower than those for the Chinese norms. The structural equations modeling revealed that the total effects coefficient of demographic characteristics on mental health was 0.68, and that of personality was 0.60. There were direct influences between demographic characteristics and personality characteristics, demographic characteristics and mental health, and personality characteristics and mental health; the standardized path coefficients were 0.60, 0.50, and 0.30, respectively. Smoking, drinking, and neuroticism were the main factors affecting the mental health of bus drivers; and the standardized path coefficients were 0.56, 0.54, and 0.90 respectively. CONCLUSIONS: In this study, bus drivers were significantly more extroverted, psychotic, and neurotic than the general population; and they were more likely to suffer from somatization and phobia. The mental health of bus drivers was associated with some demographic and personality characteristics in the study. These findings provide a theoretical basis for the selection of bus drivers and intervention measures for drivers with high-risk driving tendencies, which is conducive to better guide road traffic safety construction and reduce the occurrence of road traffic injuries.

The Efficacy of Cognitive Training for Elderly Chinese Individuals with Mild Cognitive Impairment.

The age of the population is shifting toward the elderly range, which may lead to an increased risk of mild cognitive impairment (MCI). The aims of this study are to evaluate the cognitive function in elderly people using the Montreal Cognitive Assessment (MoCA), to identify the relationship between cognitive function and different characteristics, and to evaluate the efficacy of the intervention after six months of cognitive training. In this study, we included 2886 subjects aged ≧60 years in the baseline survey, and 140 subjects with MCI who participated in the baseline survey were randomly divided into an intervention group (N = 70) and a control group (N = 70). The control group was not provided any intervention measures, and the intervention group was administered cognitive training. The education level, monthly income, sleep time, exercise time, reading times, and time spent engaging in community activities and performing housework were positively correlated with MoCA scores, but age was negatively correlated with MoCA scores. The total MoCA score of the intervention group increased from 19.77 ± 2.24 points to 21.09 ± 2.20 points after six months of cognitive training, but the score of the control group decreased from 20.41 ± 2.10 points to 19.17 ± 2.57 points. The two-way repeated-measures ANOVA revealed a very significant effect of the interaction between time and cognitive training on the total MoCA score. Seventeen participants in the intervention group improved to normal levels, and no participants progressed to dementia after six months of cognitive training. Thus, the efficacy of the intervention was statistically significant. Our study concludes that older age is associated with a cognitive decline. Factors that are more likely to protect against cognitive decline included a higher education level and monthly income, sufficient sleep time, regular physical exercise and reading, frequently engaging in community activities, and continuing to perform housework. Moreover, the cognitive training intervention is effective and may help to decrease the deterioration of cognitive function in patients with MCI, and the interaction between intervention time and cognitive training significantly improves cognitive function.

H2A and Ca-L-hipposin gene: Characteristic analysis and expression responses to Aeromonas hydrophila infection in Carassius aurutus.

Antimicrobial peptide is an important component of the host innate immune system and thus serves a crucial function in host defense against microbial invasion. In this study, H2A and derived antimicrobial peptide Ca-L-hipposin were cloned and characterized in Carassius aurutus. The gene H2A full-length cDNA is 908 bp and includes a 5'-terminal untranslated region (UTR) of 55 bp and a 3'-terminal UTR of 466 bp with a canonical polyadenylation signal sequence AATAA, as well as an open reading frame (ORF) of 387 bp encoding a polypeptide of 128 amino acids, with a molecular weight of 13.7 kDa, an isoelectric point of 10.7, and 94% homology with Danio rerio H2A. The secondary structure of H2A includes the α-spiral with 51 amino acids with a composition ratio of 39.8%, as well as a ß-corner with 15 amino acids in a composition ratio of 11.7%. The online software ExPaSy predicted that a peptide sequence with 51 amino acids from the 2nd to 52nd amino acids in histone H2A can be produced through hydrolization by protease chymotrypsin, which indicates a difference of only three amino acids, compared with the antimicrobial peptide hipposin in Hippoglossus hippoglossus with a homology of 94%. Ca-L-hipposin includes 51 amino acids with a molecular weight of 5.4 kDa and an isoelectric point of 12.0, the secondary structure of which contains an α-helix of 17 amino acids accounting for 33.3% and a ß-corner of 8 amino acids accounting for 15.7%. H2A was extensively expressed in the mRNA levels of various tissues, with higher expression levels in kidney and spleen. After C. aurutus was challenged with Aeromonas hydrophila, the mRNA expression levels of H2A were upregulated in the kidney, spleen, and liver. H2A serves an important function in the defense against the invasion of A. hydrophila. In addition, sequence characteristics reveal that Ca-L-hipposin could be a potential antimicrobial peptide for use in killing pathogenic bacteria in aquaculture.

Effects of copper exposure on the hatching status and antioxidant defense at different developmental stages of embryos and larvae of goldfish Carassius auratus.

This study aims to assess the effects of copper exposure on hatching status and antioxidant defense at different stages of embryos and larvae of goldfish Carassius auratus. In this study, day-old embryos were randomly grouped after fertilization and then exposed to copper concentrations of 0, 0.1, 0.4, 0.7, and 1.0mgL(-1). Copper-exposed fish embryos were sampled every 24h to determine superoxide dismutase (SOD), and catalase (CAT) activities, as well as malondialdehyde (MDA) content. In addition, cumulative mortality and larval deformity were also investigated. The findings showed that cumulative mortality and larval deformity rate increased gradually with copper concentration increase. SOD and CAT activities were inhibited at higher copper concentrations. At a lower concentration (0.1mgL(-1)), SOD activity increased in larvae, whereas CAT activity showed no significant change (p>0.05). MDA, as the lipid peroxidation product, gradually accumulated in embryos and larvae with increasing copper concentration and the extension of exposure time. At 0.4mgL(-1) and more, copper toxicity was shown in embryos and larvae. In conclusion, copper-exposed effects on hatching status and antioxidant defense in C. auratus embryos and larvae showed concentration- and time-dependent patterns. The biochemical parameters in this study can be used as effective indicators for evaluating the responses of copper-exposed fish embryos. In addition, this study demonstrates that 0.4mgL(-1) copper (corresponding to 1mgL(-1) copper sulfate), used to kill parasites in aquaculture, is not safe concentration, because it can result in toxicity to larvae. Therefore, the copper concentration to kill pathogen should be less than 0.4mgL(-1) for C. auratus.

Effect of lifestyle intervention on non-alcoholic fatty liver disease in Chinese obese children.

AIM: To investigate the effect of lifestyle intervention on non-alcoholic fatty liver disease (NAFLD) in Chinese obese children. METHODS: Seventy-six obese children aged from 10 to 17 years with NAFLD were enrolled for a one-month intervention and divided randomly into three groups. Group1, consisting of 38 obese children, was an untreated control group without any intervention. Group 2, consisting of 19 obese children in summer camp, was strictly controlled only by life style intervention. Group 3, consisting of 19 obese children, received oral vitamin E therapy at a dose of 100 mg/d. The height, weight, fasting blood glucose (FBG), fasting serum insulin (FINS), plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TCHO) and homeostasis model assent-insulin resistance (HOMA-IR) were measured at baseline and after one month. All patients were underwent to an ultrasonographic study of the liver performed by one operator who was blinded to the groups. RESULTS: The monitor indices of BMI, ALT, AST, TG, TCHO and HOMA-IR were successfully improved except in group 1. BMI and ALT in group 2 were reduced more significantly than in group 3 (2.44 +/- 0.82 vs 1.45 +/- 0.80, P = 0.001; 88.58 +/- 39.99 vs 63.69 +/- 27.05, P = 0.040, respectively). CONCLUSION: Both a short-term lifestyle intervention and vitamin E therapy have an effect on NAFLD in obese children. Compared with vitamin E, lifestyle intervention is more effective. Therefore, lifestyle intervention should represent the first step in the management of children with NAFLD.